UAMS.EDU

Graduate Molecular Cell Biology

Molecular Cell Biology – MBIM 6104

The goals of this course are to develop a better understanding of the molecular mechanisms underlying basic processes in cell biology.  This will be achieved by discussing in depth experimental studies that lead to proposed mechanistic models.  In addition, these studies will introduce you to potential experimental approaches to attack scientific questions.  We encourage you to critically evaluate the experiments and to incorporate this information with that from your previous or ongoing courses and research activities.

Prerequisites:  Prior course in cell biology or permission of instructor.  Biochemistry / Gene Expression are recommended.  The course will be taught with the assumption that you have had courses in these areas.

Course Instructors:

Marie Chow (Biomed 1 521, ChowMarie@uams.edu)
Paul Gottschall (Biomed 2-B341C-2, PEGottschall@uams.edu)
Thomas Kelly (Cancer Institute 318, KellyThomasJ@uams.edu)
Vladimir Lupashin (Biomed 2 261-2, VVLupashin@uams.edu)
Angus MacNicol (Cancer Institute 310, Angus@uams.edu)
Usha Ponnappan (Biomed 1 506G, PonnappanUsha@uams.edu)
Brian Storrie (Biomed 2 262-2, StorrieBrian@uams.edu)
Dan Voth (Biomed 1 506, DVoth@uams.edu)

Course structure:  The course will be 4 credit hours; Class meetings: Tues/Fri from 9:30-11:30 in Physiology Conference Room 242-2, Biomed II.  The course will be a combination of lectures, weekly written work or problem sets and class discussions.

Texts:  There is no required text.  Molecular Cell Biology by Lodish et al is one of several textbooks that can be used for background information.  Virtually all of the assigned readings will be on specific papers germane to the topic being discussed.

Written assignments:  For some of the sections, there will be problem sets.  For other sections there will be written assignments.

Grading:

1.  In-Class Paper Discussions (30%)
2.  Problem sets / written class assignments (30%)
3.  Exams (20% – 1st, 20% – 2nd)

Slides and Readings are located HERE

General outline and topic organization:

Class Period

Faculty

Class Period

General Topics
Friday  Aug 23

Lupashin/Storrie

1

Course Organization / Introduction:  Using Microscopy in Cell Biology
Tuesday Aug 27

Lupashin

2

ER transport – translocation and retro-translocation
Friday  Aug 30

3

COPII-dependent sorting at ER exit
Tuesday Sept 3

4

Rab and Arf proteins
Friday  Sept 6Tuesday Sept 10

5

6

Vesicular tethersSNARE-dependent membrane fusion
Friday  Sept 13         Storrie       7 Organelle Assembly and Function – the Golgi Apparatus as  the prototype example
Tuesday Sept 17

8

Friday    Sept 20

9

Tuesday Sept 24

10

Friday    Sept 27

Voth

11

Endosomal pathways
Tuesday Oct 1

12

Phagolysosomes
Friday Oct 4

13

Apoptosis, Autophagy and signaling
Tuesday Oct 8

Chow

14

Nuclear transport – structure of nuclear envelope / pores
Friday Oct 11

15

Getting things in and out: protein and RNA import/export;
Tuesday Oct 15

16

Translational Control;
Friday Oct 18

17

RNAi; miRNAs, siRNAs; miRNA processing and function
Tuesday Oct 22      18 Take home exam – (Pick up on Tuesday,  Return on Thursday,  9AM)
Friday Oct 25

Ponnappan

19

Signal Transduction – coordination of signaling –   specificity of output despite using common shared elements;
Tuesday Oct 29

20

Integration of distinct signaling pathways
Friday  Nov 1

21

Antagonism of signaling pathways
Tuesday Nov 5

22

Signaling pathways;
Friday    Nov 8

Kelly

23

Cytoskeleton
Tuesday Nov 12

24

Cytoskeleton
Friday    Nov 15

25

Cell Adhesion
Tuesday Nov 19

Gottschall

26

Extracellular matrix environmental sensing and cell-cell communication
Friday    Nov 22

27

Tuesday Nov 26

28

Friday    Nov 29 Thanksgiving week no class
Tuesday Dec 3

MacNicol

29

Control Cell Cycle and Cell Division
Friday    Dec 6

30

Stem Cell Biology
Tuesday Dec 10

31

Dec 13-15

Exam

Take home exam Pick up on Friday, December 13, 2013. Return  on Monday, December 16, 2013 at 9:00 AM.
Holidays:
Labor Day Sept 2 (Monday);
Veterans Day Nov. 11 (Monday);
Thanksgiving Nov. 28, 29