Brian Storrie, Ph.D.Brian Storrie, Ph.D.

Ph.D., California Institute of Technology
Office (501) 526-7418
Lab:   (501) 526-7417

Recycling pathways are a key to unraveling the interconnected problems of Golgi apparatus assembly and drug targeting.  The Golgi apparatus is the central subcellular organelle within the secretory pathway.  Surprisingly, the organized stack structure of Golgi cisternal membranes is dynamically unstable; cisternal Golgi apparatus membrane proteins continuously cycle to the endoplasmic reticulum (ER) and back.  Retrograde trafficking from the Golgi apparatus to the ER is independent of any known coat protein and inducible by rab33b, a medial rab protein, and rab6a/6a’, two rab proteins found in the trans Golgi apparatus/trans Golgi network.  Rab6a and rab6a’ appear to be redundant to one another while rab33b acts independently of rab6a/a’.  Induced Golgi protein recycling to the ER in microtubule-dependent and likely motor protein driven.  As revealed by the outcome of an ER-exit block, constitutive Golgi protein recycling is microtubule-independent and little influenced by levels of GDP-restricted rab proteins sufficient to strongly inhibit induced recycling.  The recycling of Golgi apparatus proteins to the ER implies that the Golgi apparatus may be evolutionarily a derivative of the ER.  In vivio, the Golgi apparatus can assemble de novo from the ER in a staged process in which membrane proteins and matrix dynamically nucleate sites onto which Golgi glycosyltransferases and glycosidases add later.  We find the B subunit of Shiga toxin which itself is non-toxic is an effective vector for the delivery of photosensitizers to the Golgi apparatus.  Vector delivered photosensitizers are much more effective in cell killing than those delivered by bulk transfer processes.  Current work is aimed at characterizing 1) the molecular mechanisms of constitutive and rab induced Golgi protein recycling, 2) the involvement of various gene products in Golgi assembly in vivo, and 3) the potential value of the Golgi apparatus as a target for vector carried photosensitizer delivery.

Representative Publications:

McBride EL, Rao A, Zhang G, Hoyne JD, Calco GN, Kuo BC, He Q, Prince AA, Pokrovskaya ID, Storrie B, Sousa AA, Aronova MA, Leapman RD., Comparison of 3D cellular imaging techniques based on scanned electron probes: Serial block face SEM vs. Axial bright-field STEM tomography., J Struct Biol. 2018 Jun;202(3):216-228. doi: 10.1016/j.jsb.2018.01.012. Epub 2018 Feb 1.

Krokowski D, Guan BJ, Wu J, Zheng Y, Pattabiraman PP, Jobava R, Gao XH, Di XJ, Snider MD, Mu TW, Liu S, Storrie B, Pearlman E, Blumental-Perry A, Hatzoglou M., GADD34 Function in Protein Trafficking Promotes Adaptation to Hyperosmotic Stress in Human Corneal Cells., Cell Rep. 2017 Dec 5;21(10):2895-2910. doi: 10.1016/j.celrep.2017.11.027.

Goud B, Liu S, Storrie B., Rab proteins as major determinants of the Golgi complex structure., Small GTPases. 2018 Mar 4;9(1-2):66-75. doi: 10.1080/21541248.2017.1384087. Epub 2018 Jan 29.

Banerjee M, Joshi S, Zhang J, Moncman CL, Yadav S, Bouchard BA, Storrie B, Whiteheart SW., Cellubrevin/vesicle-associated membrane protein-3-mediated endocytosis and trafficking regulate platelet functions., Blood.2017 Dec 28;130(26):2872-2883. doi: 10.1182/blood-2017-02-768176. Epub 2017 Sep 20.

Kamykowski JA, Storrie B., Managing the Introduction of Super-Resolution Microscopy into a Core Facility., Methods Mol Biol. 2017;1663:15-19. doi: 10.1007/978-1-4939-7265-4_2

Storrie B, Whiteheart SW., Editorial: Platelet Secretion., Platelets. 2017 Mar;28(2):107. doi: 10.1080/09537104.2016.1277676.<

Siegel N, Lupashin V, Storrie B, Brooker G., High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers., Nat Photonics. 2016 Dec;10:802-808. doi: 10.1038/NPHOTON.2016.207. Epub 2016 Nov 14.

Yadav S, Storrie B., The cellular basis of platelet secretion: Emerging structure/function relationships., Platelets. 2017 Mar;28(2):108-118. doi: 10.1080/09537104.2016.1257786. Epub 2016 Dec 23.

Yadav S, Williamson JK, Aronova MA, Prince AA, Pokrovskaya ID, Leapman RD, Storrie B., Golgi proteins in circulating human platelets are distributed across non-stacked, scattered structures., Platelets. 2017 Jun;28(4):400-408. doi: 10.1080/09537104.2016.1235685. Epub 2016 Oct 18.

Storrie B., A tip of the cap to procoagulant platelets., Blood.  2016 Sep 29;128(13):1668-9. doi: 10.1182/blood-2016-08-730622.

Liu S, Majeed W, Kudlyk T, Lupashin V, Storrie B., Identification of Rab41/6d Effectors Provides an Explanation for the Differential Effects of Rab41/6d and Rab6a/a’ on Golgi Organization.,    Front Cell Dev Biol. 2016 Mar 1;4:13. doi: 10.3389/fcell.2016.00013. eCollection 2016

Siegel N, Storrie B, Bruce M, Brooker G., CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)., Proc SPIE Int Soc Opt Eng. 2015 Feb 7;9336. pii: 93360S. Epub 2015 Mar 11.

Pokrovskaya ID, Aronova MA, Kamykowski JA, Prince AA, Hoyne JD, Calco GN, Kuo BC, He Q, Leapman RD, Storrie B., STEM tomography reveals that the canalicular system and α-granules remain separate compartments during early secretion stages in blood platelets., J Thromb Haemost. 2016 Mar;14(3):572-84. doi: 10.1111/jth.13225. Epub 2016 Feb 4

Ouseph MM, Huang Y, Banerjee M, Joshi S, MacDonald L, Zhong Y, Liu H, Li X, Xiang B, Zhang G, Komatsu M, Yue Z, Li Z, Storrie B, Whiteheart SW, Wang QJ., Autophagy is induced upon platelet activation and is essential for hemostasis and thrombosis., Blood. 2015 Sep 3;126(10):1224-33. doi: 10.1182/blood-2014-09-598722. Epub 2015 Jul 24.

Storrie B., Defective platelet autocrine signaling in HPS., Blood. 2015 Mar 5;125(10):1515-6. doi: 10.1182/blood-2015-01-616284.

Liu S, Storrie B., How Rab proteins determine Golgi structure., Int Rev Cell Mol Biol. 2015;315:1-22. doi: 10.1016/bs.ircmb.2014.12.002. Epub 2015 Feb 7.

MacDonald L, Baldini G, Storrie B., Does super-resolution fluorescence microscopy obsolete previous microscopic approaches to protein co-localization?, Methods Mol Biol. 2015;1270:255-75. doi: 10.1007/978-1-4939-2309-0_19.

Hickman DA, Syal G, Fausther M, Lavoie EG, Goree JR, Storrie B, Dranoff JA., MCP-1 downregulates MMP-9 export via vesicular redistribution to lysosomes in rat portal fibroblasts., Physiol Rep. 2014 Nov 20;2(11). pii: e12153. doi: 10.14814/phy2.12153. Print 2014 Nov 1.

Majeed W, Liu S, Storrie B., Distinct sets of Rab6 effectors contribute to ZW10–and COG-dependent Golgi homeostasis., Traffic. 2014 Jun;15(6):630-47. doi: 10.1111/tra.12167. Epub 2014 Apr 11.

Liu S, Hunt L, Storrie B., Rab41 is a novel regulator of Golgi apparatus organization that is needed for ER-to-Golgi trafficking and cell growth., PLoS One. 2013 Aug 6;8(8):e71886. doi: 10.1371/journal.pone.0071886. Print 2013.

Micaroni M, Stanley AC, Khromykh T, Venturato J, Wong CX, Lim JP, Marsh BJ, Storrie B, Gleeson PA, Stow JL., Rab6a/a’ are important Golgi regulators of pro-inflammatory TNF secretion in macrophages., PLoS One. 2013;8(2):e57034. doi: 10.1371/journal.pone.0057034. Epub 2013 Feb 21.

Liu S, Storrie B., Are Rab proteins the link between Golgi organization and membrane trafficking? Cell Mol Life Sci. 2012 May 13. [Epub ahead of print] Related citations

Garg TK, Szmania SM, Khan JA, Hoering A, Malbrough PA, Moreno-Bost A, Greenway AD, Lingo JD, Li X, Yaccoby S, Suva LJ, Storrie B, Tricot G, Campana D, Shaughnessy Jr JD Jr, Nair BP, Bellamy WT, Epstein J, Barlogie B, van Rhee F., Highly activated and expanded natural killer cells for multiple myeloma immunotherapy. Haematologica. 2012 Mar 14. [Epub ahead of print]. Free Article. Related citations

Storrie B, Micaroni M, Morgan GP, Jones N, Kamykowski JA, Wilkins N, Pan TH, Marsh BJ Electron Tomography Reveals Rab6 Is Essential to the Trafficking of trans-Golgi Clathrin and COPI-Coated Vesicles and the Maintenance of Golgi Cisternal Number. Traffic. 2012 May;13(5):727-44. doi: 10.1111/j.1600-0854.2012.01343.x. Epub 2012 Mar 14. Related citations

Lisauskas T, Matula P, Claas C, Reusing S, Wiemann S, Erfle H, Lehmann L, Fischer P, Eils R, Rohr K, Storrie B, Starkuviene V., Live-cell assays to identify regulators of ER-to-Golgi trafficking. Traffic. 2012 Mar;13(3):416-32. doi: 10.1111/j.1600-0854.2011.01318.x. Epub 2012 Jan 3. Related citations

Kamykowski J, Carlton P, Sehgal S, Storrie B.,Quantitative immunofluorescence mapping reveals little functional coclustering of proteins within platelet α-granules. Blood. 2011 Aug 4;118(5):1370-3. Epub 2011 May 26. Related citations

Link to Dr. Storrie at PubMed