Research Focus

Research concentrates on structural and biochemical approaches to how platelet activation leads to bleeding cessation, i.e., hemostasis on the one hand, and thrombosis, i.e., bleed vessel occlusion, on the other hand. Circulating platelets survey the circulation for damage and respond to various chemical and mechanical cues. Our goal is to characterize how these cues translate into granule secretion and platelet aggregation that can seal a wound hole or excessive response seal a blood vessel.  The laboratory uses a combination of imaging, genetic and in vitro biochemical approaches to solve structural problems in hemostasis and thrombosis.

 

Key Publications

Pokrovskaya ID, Tobin M, Desai R, Joshi S, Kamykowski JA, Zhang G, Aronova MA, Whiteheart SW, Leapman RD, Storrie B. Canalicular system reorganization during mouse platelet activation as revealed by 3D ultrastructural analysis.  Platelets. 2020 Jan 31:1-8. doi: 10.1080/09537104.2020.1719993. Online ahead of print.

Pokrovskaya ID, Joshi S, Tobin M, Desai R, Aronova MA, Kamykowski JA, Zhang G, Whiteheart SW, Leapman RD, Storrie B. SNARE-dependent membrane fusion initiates α-granule matrix decondensation in mouse platelets. Blood Adv. 2018 Nov13;2(21):2947-2958.  doi: 10.1182/bloodadvances.2018019158.

Yadav S, Storrie B. The cellular basis of platelet secretion: Emerging structure/function relationships. Platelets. 2017 Mar;28(2):108-118. doi: 10.1080/09537104.2016.1257786. Epub 2016 Dec 23.

Kamykowski J, Carlton P, Sehgal S, Storrie B. Quantitative immunofluorescence mapping reveals little functional coclustering of proteins within platelet α-granules. Blood. 2011 Aug 4;118(5):1370-3. doi: 10.1182/blood-2011-01-330910. Epub 2011 May 26.

Sehgal S, Storrie B. Evidence that differential packaging of the major platelet granule proteins von Willebrand factor and fibrinogen can support their differential release. J Thromb Haemost. 2007 Oct;5(10):2009-16. doi: 10.1111/j.1538-7836.2007.02698.x. Epub 2007 Jul 23.

 

Projects

  1. Repatterning thrombus structure through drug and gene manipulation to promote hemostasis and control thrombosis
  2. Mapping granule scaffolding protein effects in wild type and mutant mice
  3. Probing for possible structural defect in platelets from COVID-19 patients – discovery science with major analytical aspects of project being done at NIBIB, NIH

 

Grant Funding (Last Five Years)

NIH funding

Present

NIH subaward through University of Kentucky

Past

2013/08/01-2019/08/31

R56/R01 HL119393 National Heart, Lung and Blood Institute (NHLBI)

Storrie, Brian  (PI)

Structure/Function Determinants of Platelet Granule Secretion

 

2013/08/01-2018/04/30

U54 GM105814, National Institute of General Medical Sciences (NIGMS)

Storrie, Brian (PI, contact investigator)

Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function

 

2015/07/01-2017/06/30

S10 OD0618065

Storrie, Brian  (PI)

Super-Resolution Light Microcopy at University of Arkansas for Medical Sciences

Role:  (PI)

This grant funds the purchase of a Zeiss Elyra PS.1 super-resolution light microscope that supports TIRF, 3D-SIM and PALM/STORM localization microscopy.

 

2011/09/30-2016/08/31

R01 GM09296, National Institute of General Medical Sciences (NIGMS)

Storrie, Brian  (PI)

Role of Rab Proteins in Golgi Apparatus Structure and Function

 

Students/Postdocs (past and present 10 years)

Postdoc

Shilpi Yadav

Waqar Majeed

Shijie Liu

Laura MacDonald

Graduate students

Lindsey James Dickson

Andrew Prince

Siddharth Sehgal

Tregei Starr

Post-baccs at NIBIB (joint supervision with Leapman laboratory)

Joshua Cohen

Kenny Ling and Nesh Vedenaparti

Michael Tobin and Rohan Desai

Amith Rao and Emma McBride

Gina Calco, Josh Hoyne, and Brian Kuo

 

Lab personnel: staff, students and postdocs (current)

Irina Pokrovskaya, research associate III